Fig. 4

uPA-sEVs-siSrc effectively eliminate senescent stromal cells in vitro. (A, B) SA-β-gal staining (A) and quantitative analysis (B) of senescent HFF-1 and HMEC-1 cells with different treatments. Scale bar = 100 μm. (C, D) P21 staining (C) and quantitative analysis (D) of senescent HFF-1 and HMEC-1 cells with different treatments. Scale bar = 50 μm. (E, F) γH2AX staining (E) and quantitative analysis (F) of senescent HFF-1 and HMEC-1 cells with different treatments. Scale bar = 25 μm. Data are displayed as the mean ± SD (n = 3). ****p<0.0001