Fig. 4

ePL injection changes pharmacokinetics of AAV9 and nanoparticles and acts on neutralizing antibodies. a qPCR analysis of blood from AAV9.CMV-GFP injected mouse over 30 h with and without ePL. b Ear imaging IVM setup. Intravital microscopy (IVM) enabled blood clearance quantification of model latex nanoparticles with and without ePL pre-treatment. c ePL or vehicle PBS was administered i.v. to C57BL/6 mice 15 min prior to injection of latex fluorospheres (green) via catheter. Background staining was accomplished after injection of rhodamine dextran (red). A representative blood vessel imaging is depicted. A white rectangle is shown as a typical region of interest in which signal over time was quantified. d Quantification of the fluorescence signal over time from IVM experiments. e Lec2 cell transduction assays in the presence of AAV9-injected mouse serum with and without ePL injection. AAV9.CMV.Luc was used to transduce the cells and luminescence values were normalized to total protein content. f Lec2 transduction assays as in e, but with ePL added directly into cell culture media