Fig. 3

Cellular uptake and in vitro tumor therapy. (a) Uptake of 4T1 cells incubated with 50 µg/mL FessMOF-PEG@FITC at different time points. (b) Cell viability of 4T1 cells after different treatments. (c) Cell viability of 4T1 cells cocultured with FessMOF-PEG and 10 µM Ferrostatin-1 (ferroptosis inhibitor), 10 µM 3-Methyladenine (autophagy inhibitor), 20 µM Z-VAD-FMK (apoptosis inhibitor), and 20 µM vitamin E (antioxidant) treatments for 24 h. (d) Colony formation ability of 4T1 cells under different inhibitors. (e) The levels of GSH in the cells upon different treatments. All data were presented as mean ± standard deviation. Statistical differences were calculated using two-tailed Student’s t test. Differences were considered significant when the p-value was less than or equal to 0.05. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001