Fig. 3

Generation of gene-disrupted T cells and identification the expression of CAR on the surface of human T cells. (A) Schematic presentation of gene-disrupted T cells generation, Cas9/sgRNA RNP complexes were electroporated into T cells. (B) The TCR and HLA double knockout rate of T cells was 32.27% after using Cas9/sgRNA RNP complexes electroporation. (C) Schematic presentation of mutated DNA sequences of TRAC (left) and B2M (right) in the TCR and HLA knock-out T cells. (D-E) Anti-MM CARs were efficiently expressed on the surface of human gene-disrupted T cells detected by immunofluorescence assay (D) and flow cytometry (E). Scale bar 5 μm