Fig. 6

Effects of Nano-TiO2 on nitrogen metabolism in PSNPs-stressed maize plants. (A) the transcription levels of genes associated with nitrogen metabolism. Color scale denotes log2(fold-change). NRT: Nitrate transporter; NR: Nitrate reductase; GS: Glutamine synthetase; GOGAT: Glutamate synthetase; GDH: Glutamate dehydrogenase. Nano-TiO2 effects on nitrate (NO3−) content (B), NR activity (C), ammonia (NH4+) content (D), GDH activity (E), GS activity (F), GOGAT activity (G), soluble protein content (H), free amino acid content (I), and protease activity (J). C: Control; Ti: Nano-TiO2; P + Ti: PSNPs + Nano-TiO2. Different letters indicate statistically significant differences (n = 4, P < 0.05)