Fig. 2

The binding of ELPs to LLC and 4T1 cells. LLC (a) and 4T1 (b) cells were incubated with fluorescein isothiocyanate (FITC)-labelled ELPs (0.5 µM) at 37 °C for 1 h. The percentage of cell-binding was analyzed through flow cytometry (n = 3). Graphical bars on the right represent the percentage of ELP-bound cells as the mean ± standard deviation (SD) of data obtained from three separate experiments performed in triplicate. ***P < 0.001 (one-way analysis of variance (ANOVA)) indicates significant differences for Tat-E60, A86 and Tat-A86 compared with untreated control. c LLC cells were treated with 0.5 µM of the respective fluorescein isothiocyanate (FITC)-labelled polypeptides at 37 °C for 1 h and observed under confocal laser microscopy. Cell membranes and nuclei were stained with Wheat Germ Agglutinin Alexa 594 and Hoechst, respectively. Representative confocal images from five independent experiments. Scale bar, 20 µm