Fig. 4

Physical characterization of miRNA/ELP complexes. a The turbidity profiles of the miRNA/ELP complexes (red) were assessed at 350 nm, with a temperature increase of 1 °C/min, and compared with those of naïve ELPs (black) at various temperatures. To validate the stable condensation of miRNA/ELP complexes, nanoparticle size was determined using dynamic light scattering (DLS) at 24 °C. The size parameters of ELPs alone and sizes following complex formation including Tat-E60 (b, c), A86 (d, e), and Tat-A86 (f, g) were evaluated. h TEM images of miRNA/Tat-E60, miRNA/A86 and miRNA/Tat-A86 nanocomplexes at room temperature. Scale bar = 200 nm