Fig. 7

miR-34a/ELPs induce G2/M phase arrest and cellular apoptosis. a LLC cells were transfected with miRNA-34a/ ELP, and the cell cycle distribution was analyzed 48 h post-transfection using flow cytometry after propidium iodide (PI) staining. b Flow cytometry analysis, followed by annexin V–PI staining, revealed the apoptosis percentages in LLC cells treated with different miRNA-34a/ELPs. Representative images from the flow cytometry analysis are shown. c The percentages of cell populations in the G0/G1, S, and G2/M phases were analyzed relative to the total phases. Most miRNA-34a/ELPs induced cell cycle arrest at the G2/M phase in LLC cells. Data are presented as the mean ± SD from at least three independent experiments. ***P < 0.001 indicates statistically significant difference in the miR-34a/Tat-E60, miR-34a/A86 and miR-34a/Tat-A86 -treated group compared to the control group. d The percentage of apoptotic cells increased significantly in miRNA-34a/Tat-A86-treated LLC cells compared to the control. Data are presented as the mean ± SD for relative apoptosis normalized to control cells for three independent experiments. Columns represent the mean of three separate experiments, while bars represent the SD. **P < 0.01, ***P < 0.001 as determined by ANOVA test, miRNA-34a/Tat-E60 vs. miRNA/miRNA-34a/A86, and miRNA-34a/Tat-E60 vs. miRNA-34a/Tat-A86