Fig. 8

Growth inhibition assay using the LLC spheroids. a–d LLC spheroids were treated with different miRNA/ELP complexes, featuring varying miRNA-34a concentrations (5 or 10 μM) encapsulated within different ELPs. The time-dependent increase in the diameter of LLC spheroids was monitored for 10 days using bright-field imaging. Representative images of spheroid treated with miRNA/ELP complexes are presented. LLC spheroids cultured in Roswell Park Memorial Institute (RPMI) medium served as a control. Scale bar, 500 μm. Growth curve of LLC spheroids after various treatments. **P < 0.01, Tat-A86/miRNA-34a treated vs. control group from day 6 onwards. e, f Cell viability assay of the differently sized spheroids (day 3 and day 6) using live/dead staining with ethidium homodimer-1 and calcein AM. Viable cells are depicted in green, while nonviable cells are shown in red. Scale bar, 500 μm