Fig. 10

In vivo wound closure assays. (A) Schematic diagram of mice model with full-thickness skin wound repaired by CS-PE-Qu hydrogel film (B) Hemolysis rate and hemolysis test digital images (inset) of control, CS, CS-Qu, CS-blank PE, CS-PE-Qu films, and positive control (from left to right) (C) Representative digital photographs of wounds treated with medical gauze (control group), CS, CS-Qu, CS-blank PE, and CS-PE-Qu films at different time points (D) The wound closure ratio at different time points (n = 4). (E) H&E staining images of the normal skin (NS) and wound tissues treated with CS, CS-Qu, CS-blank PE, and CS-PE-Qu films on day 10. The right panel is enlarged images of dotted black boxes in the left panel. The red dotted lines show re-epithelization, S: scab, E: epidermis, D: dermis, H: hyperplastic, PK: parakeratosis, F: fibroblast, HF: hair follicle, C: collagen, I: inflammatory cells. Values were expressed as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001 vs. controls at their respective time point. ^†P < 0.05 and ^††P < 0.01 vs. CS-blank PE at respective time points