Fig. 3
From: Combined SERS-Raman screening of HER2-overexpressing or silenced breast cancer cell lines
Raman classification of breast derived cell lines expressing different HER2 levels. (A) Median + IQR spectra of SKBR3 (lime), MCF10A (green) and MDA-MB-468 (blue), with PCA loading expressing high variance, black lines (y is offset for clarity). Vertical dash lines are single peaks of interest; cyan vertical bands are relevant biological bands in cell classification. (B) 3D score plot of PC2-4 scores demonstrating the classification achieved for SKBR3, MCF10A and MDA-MB-468 cells using 3 components. (C) Box-plot of the distribution for each cell line of PC4 scores. The asterisks represent Wilcoxon nonparametric test results with p < 0.0001 when ****. (D-E) LDA supervised classification with the separation achieved using the latent variables 1 and 2, and the misclassification rate, respectively