Fig. 3

Volumetric US/PA images of an excised brain from a mouse treated with the intracerebral imaging and delivery approach. The mouse received intravenous injection of PFCnDs-760 and PFCnDs-1064 and was treated with PFCnD-induced BBB opening. The animal was euthanized four hours after nanodroplet injection. PA images used imaging wavelengths of (A, B) 760 nm and (C, D) 1064 nm. (E, F). A merged image of the signals acquired at both wavelengths is shown. Images show the localization of PFCnDs to the treated (right) side of the brain. The image perspectives include transverse (A, C, E) and corner-cut (B, D, F) views of the treated (right) side of the brain