Fig. 2

ROS scavenging effect of FSZ nanomaterials. (A) PC12 cells were treated with different dose (0, 5, 10, 20, 40, 80, and 160 μg/ml) of SSSe, SSe, Se, ZIF-8, SSSe@ZIF-8, SSe@ZIF-8 and Se@ZIF-8 for 48 h. CCK-8 assay was used to determine cell viabilities. (B-C) The concentration-dependent analysis (0, 5, 10, 20, 40 and 100 μg/ml) of SSSe, ZIF-8, SSSe@ZIF-8, SSe@ZIF-8 and Se@ZIF-8 was co-cultured with 100 μM H₂O₂, the TMB assay was performed to detect •OH elimination. (D) The time-dependent analysis of the absorbance of TMB at 645 nm due to •OH elimination. (E-F) The concentration-dependent investigation of ABTS•+ in the presence of SSSe, ZIF-8, SSSe@ZIF-8, SSe@ZIF-8 and Se@ZIF-8 (0, 5, 10, 20, 40 and 100 μg/ml). (G) The time-dependent investigation of the absorbance of ABTS•+ at 734 nm. (H) SSe@ZIF-8 was coated with Ferrostatin 1 to obtained FSZ nanoparticles. (I) Ferrostatin 1 release rate detection. (J) FSZ nanoparticles were co-cultured with 5%H₂O₂ and detected by TEM every 2 h. (K) PC12 cells were co-cultured using 100 μM TBHP and PBS, SSSe, ZIF-8, Fer-1, SSe@ZIF-8, and FSZ nanoparticles. Intracellular ROS level was detected by DCFH-DA probes (green) and quantitated analysis (L). Data are presented as means ± SD (n = 3). Statistical analysis was performed using one-way ANOVA. *P < 0.05, **P < 0.01, and ***P < 0.001, compared with the control group. # P < 0.05 compared with the SSSe group;