Fig. 19

(A, B) RNA assembly onto a universal SNA scaffold via an enzymatic method [149]. (A): SNA is made up of an Au NP core functionalized with a DNA “anchor” oligonucleotide [1]. The DNA “bridge” (2a), another DNA oligonucleotide matching to the 3′ end of 1 (anchor). Following the hybridizing with 1, the sticky end formed by the DNA bridge (2a) is utilized to guide the hybridizing and assembly of the 5′ end of an RNA (3a) to the SNA surface. (Note: for siRNA sequences, this strand is the sense strand of a siRNA duplex). (B): By altering the sticky end sequence of the DNA bridge located at its 3′ end (2b), it is possible to match the sequence with the 5′ end of another RNA oligonucleotide (3b). This process enables the assembly of diverse RNA sequences on an SNA coated using the same DNA anchor [1]. (C) Hairpin-like design, a hairpin-like siRNA, a single RNA strand made of a duplex, and a hairpin-like region of PEG spacers, are bound to the core with high duplexing efficiency [150]. This figure was redrawn with permission from the mentioned references