Fig. 4
From: Organ-on-a-chip: future of female reproductive pathophysiological models
Mimicking the function of the fallopian tube on the chip. (A) Illustration of the human Fallopian tube, showing the longitudinal folds in cross-section at the (a) infundibulum, (b) ampulla and (c) isthmus. Reproduced with permission [50]. Copyright 2006 Oxford University Press. (B) The device features a complete separation of the apical and basolateral compartments, which allows distinct collection of secreted factors from, or introduction of exogenous factors to, the apical (luminal) and basolateral (blood circulation) compartments. Reproduced with permission [18]. (C) Effects of steroid hormone stimulation on bovine oviduct epithelial cells (BOEC) cell and cilia growth and differentiation during the luteal phase and preovulatory phase were simulated using the device in Fig. 4B. 3D reconstruction of confocal immunofluorescent images for cilia (acetylated alpha-tubulin, green), nuclei (HOECHST 33,342, blue), and actin filaments (phalloidin, red). Reproduced with permission [18]. (D) Hormonal perfusion of cultured human fallopian tube epithelial (hFTE) tissue with low (0.8 nM) versus high (2 nM) androgen conditions using a pneumatic pump to mimic polycystic ovary syndrome (PCOS) androgen exposure. Reproduced with permission [54]. Copyright 2020 Oxford University Press