Table 3 Organ-on-a-chip for modeling the pathophysiology of female reproductive system diseases
From: Organ-on-a-chip: future of female reproductive pathophysiological models
Model category | Cell types | Culture environment | Device characteristics | Culture characteristics | Significance | Year/Reference |
|---|---|---|---|---|---|---|
Placenta-on-a-Chip | λ HUVECs λ BeWo | λ Medium: HUVECs: ECM medium; BeWo: DMEM/F-12 medium; λ Temperature: 37 °C; CO2: 5% | λ The upper and lower layers with microchannels: 1.5 cm×1.5 mm×400 μm λ Transparent semipermeable membrane: 0.4 μm pores λ Device material: PDMS λ Fabrication method: soft lithography technology | λ Flow rate: 10 µl/h λ Coating: 0.1 mg/mL Collagen I | Placental inflammation model | 2018/ [114] |
Placenta-on-a-Chip | λ HUVECs λ BeWo | λ Medium: HUVECs: endothelial cell growth medium; BeWo: Ham’s F-12 K nutrient mixture + 10% FBS + 40 mg/mL gentamicin λ Temperature: 37 °C; CO2: 5% | λ Three channels: 300 μm wide and 220 μm high λ Device material: PDMS λ Fabrication method: soft lithography technology | λ Flow rate: / λ Coating: ECM gel | Placental inflammation model | 2022/ [115] |
Amnion membrane organ-on-chip | λ hiPSC | λ Medium: mTesR1 medium λ Temperature: 37 °C; CO2: 5% | λ Two parallel medium channels: 1 × 0.5 × 1.4mm3 λ The middle matrix channel: 1 × 0.2 × 1.4mm3 λ Device material: PDMS λ Fabrication method: soft lithography technology | λ Flow rate: 1 µl/h λ Coating: Matrigel | Amniotic inflammation model | 2020/ [122] |
Amnion membrane organ-on-chip | λ Primary AECs λ Primary AMCs | λ Medium: AECs: Ham’s F-12/DMEM + 10% FBS + 10 ng/ml EGF + 2 mM L-glutamine + 100 U/ml penicillin G + 100 mg/ml streptomycin; AMCs: complete DMEM-F12 medium + 5% FBS + 100 U/ml penicillin G + 100 mg/ml streptomycin λ Temperature: 37 °C; CO2: 5% | λ The first layer: 5 μm deep λ The second layer: 500 μm thick λ Device material: PDMS λ Fabrication method: soft lithography technology | λ Flow rate: / λ Coating: type IV collagen matrigel | Oxidative stress model | 2019/ [116] |
Amnion membrane organ-on-chip | λ AECs λ Decidual cells | λ Medium: AECs: Ditto; Decidual cells: Ham’s F-12/DMEM + 5% FBS + 10 ng/mL EGF + 100 U/ml penicillin G + 100 mg/ml streptomycin λ Temperature: 37 °C; CO2: 5% | λ Two microfluidic chambers: 4.75 mm×6.2 mm λ A semipermeable polycarbonate membrane λ Device material: PDMS λ Fabrication method: soft lithography technology | λ Flow rate: / λ Coating: Matrigel | Oxidative stress model | 2020/ [117] |
Feto-maternal interface organ-on-chip | λ AECs λ Decidua cells λ CTs λ AMCs | λ Medium: AECs: Ditto; Decidual cells: Ditto; CTs: Ham’s F12/DMEM + 10% FBS + 100 U/mL penicillin G + 100 mg/mL streptomycin; AMCs: DMEM-F12 medium + 5% FBS + 100U/mL penicillin G + 100 mg/mL streptomycin λ Temperature: 37 °C; CO2: 5% | λ Chamber 1: decidual cells, 250 μm in height, 3000 μm in width λ Chamber 2: fetal CTs and CMCs, 250 μm in height, 2000 μm in width λ Chamber 3: AMCs, 250 μm in height, 2000 μm in width λ Chamber 4: AECs, 250 μm in height, 600 μm in width λ Device material: PDMS λ Fabrication method: soft lithography technology | λ Flow rate: / λ Coating: type IV collagen | Maternal infection model | 2020/ [118] |
Feto-maternal interface organ-on-chip | λ Ditto | λ Ditto | λ Ditto | λ Ditto | Environmental toxin exposure model at the feto-maternal interface | 2022/ [119] |
Feto-maternal interface organ-on-chip | λ hFM_AEC λ hFM_DEC λ hFM_CTC λ hFM_AMC | λ Medium: hFM_AEC: KSFM + 30 µg/mL bovine pituitary extract + 0.1 ng/mL EGF + 0.4 mM CaCl2 + 0.5 mg/mL primocin; hFM_DEC: DMEM/F-12 + 10%FBS + 10% penicillin/streptomycin + 10% amphotericin B; hFM_CTC: DMEM/F-12 + 0.20% FBS + 0.01 mM β-mercaptoethanol + 0.5% penicillin/streptomycin + 0.3% BSA + 1× ITS-X + 2 µM CHIR99021 + 0.05 µM A83-01 + 1.5 µg/mL L-ascorbic acid + 50 ng/mL EGF + 0.08 mM VPA + 1×Revitacell; hFM_AMC: DMEM/F-12 + 5% FBS + 10% penicillin/streptomycin + 10% amphotericin B λ Temperature: 37 °C; CO2: 5% | λ Ditto | λ Ditto | Oxidative stress model at the feto-maternal interface | 2023/ [120] |
Amniotic fluid organ-on-a-chip | λ Amniotic fluid λ Human fetal brain cell | λ Medium: Human fetal brain cell: ATCC-formulated Eagle’s Minimum Essential Medium + 10% FBS λ Temperature: 37 °C; CO2: 5% | λ The first microchannel layer: 600 × 30 × 5µm3 λ The second cell culture chamber layer: 500 μm deep λ Device material: PDMS λ Fabrication method: soft lithography technology | λ Flow rate: / λ Coating: type IV collagen | Fetal neuroinflammation model | 2022/ [121] |
OvCa-Chip | λ A2780 λ HUVECs | λ / | λ The top tumor channel: 400 μm wide, 100 μm high λ The bottom vascular channel: 400 μm wide, 100 μm high; 20 mm long λ Device material: PDMS λ Fabrication method: soft lithography technology | λ Flow rate: 0.5 µL/min λ Coating: collagen-fibronectin | Ovarian cancer-vascular-blood model | 2020/ [19] |
OvCa-Chip | λ HOMECs λ A2780 | λ Medium: HOMECs: RPMI 1640 + 100 U/ml penicillin + 100 µg/ml streptomycin; A2780: RPMI 1640 + 100 U/ml penicillin + 100 µg/ml streptomycin λ Temperature: 37 °C; CO2: 5% | λ The top portion of the device was changed from a single microchannel (1 mm×1 mm×100 μm) to three parallel microchannels λ Each micropillar: 250 × 100 × 100µm3 λ Device material: PDMS λ Fabrication method: soft lithography technology | λ Flow rate: / λ Coating: collagen I | Ovarian tumor microenvironment chip | 2021/ [128] |
Endometriosis organ-on-a-chip | λ ESCs λ HPMCs | λ Medium: ESCs: DMEM + 10% FBS + 2000 u penicillin + streptomycin + glutamine; HPMCs: complete medium λ Temperature: 37 °C; CO2: 5% | λ The microchannels: 1.5 cm×300 μm×300 μm λ Device material: PDMS λ Fabrication method: / | λ Flow rate: / λ Coating: / | Pathological model of peritoneal endometriosis | 2012/ [14] |
Endometriosis organ-on-a-chip | λ 12Z cells | λ Medium: DMEM-F12 medium λ Temperature: 37 °C; CO2: 5% | λ Device features: / λ Device material: PDMS λ Fabrication method: soft lithography technology | λ Flow rate: / λ Coating: collagen hydrogel | Organotypic organ-on-a-chip luminal model for endometriosis | 2023/ [137] |