Preparation and in vivo and ex vivo studies of sirolimus nano-in-situ gel ophthalmic formulation

Table 1 Inhibitors and their main effects

Inhibitor	Concentration	Primary Action
Chlorpromazine	6 µg/mL	Inhibitor of clathrin-mediated endocytosis
Indomethacin	100 µM	Inhibitor of cell membrane caveolae-like invaginations-mediated endocytosis
Heparin	100 µg/mL	Specific inhibitor of heparan sulfate proteoglycans
Amiloride	10 µM	Specific inhibitor of macrophagocytosis
Sodium azide	0.10%	General inhibitor of endocytosis process
Hypertonic sucrose	0.45 M	Inhibitor of lattice protein-mediated endocytosis through K⁺depleting effects
Methyl-β-cyclodextrin	10 mM	Cholesterol depleting agent capable of inhibiting lipid raft/envelope cellar-dependent cytosis
Chloroquine	125 mM	Destroys endosomes and lysosomes, prevents endosomal acidification, and causes swelling of endosomes and lysosomes
Mycophenolate	10 µg/mL	Inhibits lipid raft/periplasmic caveolae-like invagination-dependent endocytosis via cholesterol chelation

ISSN: 1477-3155