Fig. 3

Antitumor activity of mNK-sEV in vivo. A Survival rates of MGC803 xenografted tumor-bearing mice that received different treatments as indicated. B Representative images of subcutaneous xenografted murine model established by MGC803 cells in BALB/c nude mice (n = 5 per experimental condition) that received PBS or mNK-sEV treatment. C Weights of the harvested tumors. D Tumor growth curves subject to different treatments. E, F Representative image of Ki-67 staining of tumors (scale bar = 100 µm). G, H TUNEL staining of tumors (scale bars = 100 µm). I Body weights of mice during in vivo efficacy study. The body weights of mice were measured every three days. Data are shown as mean ± SD. J Histological images of major organs (scale bar = 100 µm). K Weight index of major organs, including kidneys, livers, brains, lungs, and hearts from mice, which were calculated as organ weight (mg) per gram (g) of mouse body weight. *p < 0.05, **p < 0.01, ***p < 0.001