Fig. 5

mNK-sEV induced apoptosis in tumor cells. A, B JC-1 probe staining for detecting the mitochondrial membrane potential of A MGC803 and B A549 cells when treated with mNK-sEV for 24 h. C–F Western blot assays for the expression of proteins in apoptosis signaling pathway in C, D MGC803 and E, F A549 cells when treated with 100 µg/mL mNK-sEV for 24 h. G, H Cells were incubated with 100 µM zVAD-fmk for 30 min, followed by incubation with PBS or 100 µg/mL mNK-sEV for 24 h. The percentages of apoptotic cells in G MGC803 and H A549 cells were analyzed by flow cytometry (n = 3). *p < 0.05, **p < 0.01, ****p < 0.0001