Fig. 8

Blocking GNLY reduced cytotoxicity of mNK-sEV. A qRT-PCR assay for detection of cytotoxicity-related gene expression in conNK cells and mNK cells. GNLY, granulysin; PRF1, perforin 1. B Western blot analysis of GNLY expression in NK cells and NK cell-derived sEV. C, D Cells were incubated with 100 µg/mL of NK-sEV in the presence of GNLY neutralizing antibody. Flow cytometry analysis of cell apoptosis in C MGC803 and D A549 cells at 24 h (n = 3). E–H Western blot assays for the expression of proteins in apoptosis signaling pathway in E and F MGC803 and G and H A549 for 24 h. ns: no significance. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001