Fig. 3

biodistribution of mlt and characterization of hc-needle. (a) schematic representation of the mouse biodistribution experiment. (b) imaging of ra mice post-administration. (c) mice were injected as described in (b) and major organs were collected as the specified time points; the fluorescence intensity of the organs was evaluated using the in vivo imaging system. representative images are shown. (d) quantification of fluorescence intensity of the in vitro mlt release experiments. (e) analysis of serum safety indicators (alt, ast, bun, cr, sp-a, and sp-d) before and after administration. (f) appearance and schematic diagram of hc-needle. (g) schematic diagram of the hc-needle transport hydrogel. (h) the image depicting the mlt-gel remaining on the skin surface after ca- and hc-needle insertion into the muscle (the gel remaining after ca-needle insertion is indicated by the red arrow). (i) cy-5.5-labeled mlt-gel-loaded ca- and ha-needles were used to pierce the porcine tissue sample and the fluorescent signal was imaged; representative images are shown. (j) quantification of the fluorescence intensity of the experiments described in (i). (k) hc-needle for mouse (red-marked areas indicate the locations of retained needle holes). (l) macroscopic image of hc-needle insertion at mouse st36. (m) fluorescent image of mlt-gel retained at the local acupoint