Fig. 2

16-month lung presents unique cellular immunophenotype. Cellular comparison was performed of the murine lung for two age groups of mice (2- and 16-months). (A) Fold change in surface marker MFI of 16-month isolated cell types compared to average expression of the 2-month group. Boxes with Xs indicate markers that were not expressed in those cell types. (B) Multicolor flow cytometry analysis of overall CD45⁺ cell counts and CD45 median fluorescence intensity (MFI). C-F.) Flow cytometry isolated individual cell counts for alveolar macrophages (AM; C), interstitial macrophages (IM; D), CD103⁺ dendritic cells (DCs; E), and CD11b⁺ DCs (F) in the two ages. Displayed numerical results represents mean ± SD (n = 5) from untreated group results. Indicated significance is calculated via unpaired Student’s t-tests [p < 0.05 (*), 0.01 (**), 0.001 (***), < 0.001 (****)]