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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: Age-dependent changes in phagocytic activity: in vivo response of mouse pulmonary antigen presenting cells to direct lung delivery of charged PEGDA nanoparticles

Fig. 5

Aged phagocytotic cells in BALF show reduced NP uptake. After 72 h orotracheal instillation of positive (+) or negative (-) PEGDA NPs, cells isolated from BALF from 2- and 16-month mice. Representative stained images to identify nuclei (DAPI; blue), actin (Phalloidin; grey), and NP uptake (pink) were taken for 2-month (+)NP (A), 2-month (-)NP (B), 16-month (+)NP (C), and 16-month (-)NP (D). Images were taken using Biotek Cytation 5 Multimode Imager in which exposure was modified to visualize NPs in various samples. 2-month (-) NP samples (B) were at least 2x shorter in integration time than all other conditions. Scale bar represents 50 μm

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Journal of Nanobiotechnology

ISSN: 1477-3155

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