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Fig. 6 | Journal of Nanobiotechnology

Fig. 6

From: Age-dependent changes in phagocytic activity: in vivo response of mouse pulmonary antigen presenting cells to direct lung delivery of charged PEGDA nanoparticles

Fig. 6

Immunofluorescence histology highlights CD11c+cellular uptake of NPs. Frozen whole lung murine tissue was sectioned at 10 μm thickness after 72 h dosage of either positive (+; A, C,E, G) or negative (-; B, D,F, H) nanoparticles (NPs; pink) via orotracheal instillation. The tissue was stained with primary anti-hamster CD11c, secondary IgG Alexa Fluor 488 (green), and DAPI (nuclei; blue). Representative images (n = 1) represent 2-month specimens (A, B, E, F) and 16-month specimens (C, D, G, H). White arrows indicate overlap between CD11c+, NP+ cells, while pink arrows represent non-overlapping “non-localized” NPs. Images acquired using Biotek Cytation 5 Multimode Imager and exposure was modified for viewing of nanoparticles and CD11c stains in each image. Top and bottom row scale bars represent 100 μm and 25 μm, respectively

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Journal of Nanobiotechnology

ISSN: 1477-3155

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