Fig. 7
Nanoparticle uptake in aged cells changes activation profile. A.) Multicolor flow cytometry results from partial lung tissue digests after 72-hr pulmonary delivery of PEGDA NPs. Alveolar macrophages (AMs; CD45+, CD11c+, SiglecF+) and dendritic cells (DCs; CD45+, CD11c+, MHCIIhigh) were isolated from partial lung digest. AMs (B-D) and DCs (E-G) expression of CD80 (B, E), CD86 (C, F), and MHCII (D, G) were compared across NP treatments using fold change from age-appropriate UT. Data represents mean ± SD (n = 5). Indicated significance is calculated via two-way ANOVA [Tukey Test, p < 0.05 (*), 0.01 (**), 0.001 (***), < 0.001 (****)]