Fig. 4
Golgi dispersion and fragmentation by S. aureus EV infection. (A) Representative STORM images of the Golgi apparatus in epithelial keratinocyte (HaCaT) cells treated with S. aureus EVs (left) or S. epidermidis EVs (right) after 30 min of EV infection. Enlargement of the boxed region is shown at the bottom. Schematic diagrams are shown together. Red dashed line: nucleus boundary. (B) Quantitative analysis of the ultrastructural changes of the Golgi apparatus in terms of the number of Golgi fragments and the radial distribution from the center of the nucleus. (n = 10, mean ± SD) (C) 3D reconstructed HVEM images of HaCaT cells treated with S. aureus (left) or S. epidermidis (right) EVs after 30 min of EV infection at different angles, showing the fragmentation and dispersal of the Golgi apparatus by S. aureus EV infection. (D) Representative STORM images of acetylated tubulin to reveal the microtubule organizing center (MTOC) in HaCaT cells treated with S. aureus EVs (left) or S. epidermidis EVs (right). Enlargement of the boxed region is shown together at the bottom. (E) Quantitative analysis of the ultrastructural changes of the MTOC in terms of localization density and number of acetylated microtubule bundles extending from MTOC from STORM images, showing depolymerized MTOC by S. aureus EV infection. (n = 10, mean ± SD) (ns: p > 0.05, ***p < 0.001) Scale bars: (A) 5 μm (top), 1 μm (bottom), (C) 100 nm, (D) 5 μm (top), 1 μm (bottom)