Fig. 2

Cellular uptake and retention in vitro. Cellular uptake of the nanomedicine was observed by A) confocal microscopy and evaluated by B) flow cytometry. C) Confocal microscopy and D) flow cytometry showed that after laser irradiation, tumor cells exhibited enhanced absorption and retention of the nanomedicine. E) Confocal image of 4T1 tumor spheres incubated with NLG919@CF. (***p < 0.001, ****p < 0.0001)