Fig. 7

NBTXR3 + PRT + αPD1 treatment promotes a shift toward proinflammatory M1 phenotype in tumor-associated macrophages. A UMAP of macrophage subclusters. B Proportions of macrophage subclusters in irradiated tumors. C Proportions of macrophage subclusters in unirradiated tumors. D Expression of Mrc1 and Nos2 in macrophages within irradiated tumors in the groups: Control, NBTXR3 + XRT + αPD1, and NBTXR3 + PRT + αPD1. E Expression of Mrc1 and Nos2 in macrophages within unirradiated tumors in the groups: Control, XRT + αPD1, NBTXR3 + XRT + αPD1, PRT + αPD1, and NBTXR3 + PRT + αPD1. The expression levels of Mrc1 and Nos2 were analyzed using the Kruskal–Wallis test. A p-value of < 0.05 was deemed statistically significant. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. NS, not significant