Fig. 2

FliC-76 binding to S. Enteritidis and inhibition of its motility. A Binding conformation between FliC-Nb76 with FliC protein and the yellow areas are the Binding regions. B Two-dimensional graphics of FliC-Nb76 (B) interacts with FliC protein (A). C Analysis of EGFP- FliC-Nb76 protein purification by SDS-PAGE. D Determination EGFP- FliC-Nb76 by Western blot. E Observation of S. Enteritidis and EGFP-FliC-Nb-76 Binding under Laser Confocal Microscopy. F Motility analysis of S. Enteritidis on 0.4% agar LB plate by treatment with BSA and FliC-Nb76. G Observation of S. Enteritidis flagella treated with BSA and FliC-Nb76 under electron microscopy. H Statistical analysis of the movement areas of clinical resistant S. Enteritidis strains by treatment with FliC-Nb76. I Determination of minimum effective concentration of FliC-Nbs to inhibit the motility of S. Enteritidis FY-04. J Motility analysis of S. Enteritidis treated with BSA and FliC-Nb76 under Laser Confocal Microscopy. K Movement distance of S. Enteritidis treated with BSA and FliC-Nb76