Fig. 1

Synthesis and characterization of CAELN. (A) The process of synthesizing exosome-liposome hybrid nanoparticles using ultrasound, demonstrating the hybridization and fusion of the membranes of exosomes and liposomes. (B) Particle size, PDI and Zeta potential of liposomes, exosomes, CELN, and CAELN, along with their TEM images (C). (D) Images of DiD and DiI staining of liposomes and exosomes after FRET experiments, measured in a fluorescence spectrophotometer. (E) Western blotting images. (F) Agarose electropherograms after the addition of sgRNA/Cas9 plasmid at different ratios, with encapsulated sgRNA/Cas9 retained in the upwelled wells. (G) Excitation and emission wavelengths of AS1411 measured in a fluorescence spectrophotometer, along with the standard curve