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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: A reactive oxygen species-responsive hydrogel loaded with Apelin-13 promotes the repair of spinal cord injury by regulating macrophage M1/M2 polarization and neuroinflammation

Fig. 3

Apelin-13 reduces neuroinflammation by recovering macrophage polarization sequence interruption at damaged sites in SCI rats (A) At day 28 after SCI, the mRNA expression levels of pro-inflammatory cytokines TNF-α, IL-1β, IL-2 and iNOS and anti-inflammatory cytokines TGF-β1, IL-4 and IL-10 were analyzed using qRT-PCR. (B) At day 28 after SCI, MPO activity at the injured site of SCI rats was detected using an MPO fluorescence assay kit. (C-D) On day 28 after SCI, the contents of M1 macrophage marker CD16/32 and M2 macrophage marker Arg1 at the injured site of SCI rats were detected using Immunofluorescent staining (IF staining). (E) On day 28 after SCI, the protein levels of M1 macrophage marker CD16 and M2 macrophage marker Arg1 at the injured site of SCI rats were detected using Immunoblotting. n = 6 or 3, ** p < 0.01, vs. sham group; ## p < 0.01, vs. SCI + vehicle group; && p < 0.01, vs. SCI + Apelin-13 + scramble siRNA group

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Journal of Nanobiotechnology

ISSN: 1477-3155

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