Fig. 2

3T3-L1 cell-derived miR-146a-5p participates in C2C12 cell proliferation and differentiation. (a) 3T3-L1 cells were co-cultured with C2C12 cells, and the cells were grown in a transwell. (b) The expression of miR-146a-5p gene in 3T3-L1 cells following transfection with mimics and inhibitors (n = 6). (c) The expression of miR-146a-5p gene in co-cultured C2C12 cells following transfecting 3T3-L1 cells with mimics and inhibitors (n = 6). (d) CCK-8 result of co-cultured C2C12 cells (n = 9). (e, f) EdU image and statistical analyses of C2C12 cells (scale bar = 50 μm) (n = 7). (g) RT-qPCR analysis for Cyclin A2, Cyclin D1, Cyclin E1, and PCNA of C2C12 cells (n = 6). (h, i) The protein levels of Cyclin A2, Cyclin D1, Cyclin E1, and PCNA by Western blot and the statistical analyses results of C2C12 cells (n = 3). (j) RT-qPCR analysis fo MyoD, MyoG, Fbx32 and MuFR of C2C12 cells (n = 6). (k, l) The protein levels of MyHC, MyoD, MyoG, Fbx32, and MuFR by Western blot and the statistical analyses results of C2C12 cells (n = 3). (m, n) Representative muscle fiber immunofluorescent MyHC staining of C2C12 cells (scale bar = 50 μm) (n = 4). Values are presented as means ± SEM, *P <0.05, and **P <0.01, according to the non-paired Student’s t-test or one-way ANOVA between individual groups