Fig. 1

Characterization of NM-PB nanozymes and targeting capacity of NM-PB nanozymes in vitro. (A) SEM images (scale bar = 200 nm) and TEM images (scale bar = 20 nm) of PB, N-PB, M-PB, NM-PB nanozymes. (B) XRD pattern of PB nanozymes. (C) XPS spectra of PB nanozymes in the Fe 2p region. D-E) DLS and Zeta potential analysis of PB, N-PB, M-PB, and NM-PB PB nanozymes. F) The concentrations of iron measured by ICP-OES in various groups (n = 3). G) CLSM images of the neutrophil-macrophage hybrid membrane (scale bar = 10 μm); SDS-PAGE protein analysis of PB nanozymes, N, M, NM, N-PB nanozymes, M-PB nanozymes, and NM-PB nanozymes by Coomassie blue staining. H) Intracellular uptake of N-PB, M-PB, and NM-PB nanozymes (the blue indicates nucleus stained with DAPI, the red indicates N-PB, M-PB, and NM-PB nanozymes stained with Dil) (scale bar = 20 μm). *p < 0.05, ***p < 0.001, ****p < 0.0001. one-way ANOVA, Tukey’s multiple comparison test