Fig. 2
ROS scavenging activity of NM-PB nanozymes and induction of macrophage reprogramming in vitro. A-C) The scavenging effect of NM-PB nanozymes on •OH, •OOH, and H2O2. D-E) Intracellular ROS scavenging capacity of NM-PB nanoenzymes as detected by flow cytometry and CLSM (scale bar = 10 μm). F) Relative mRNA levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) in FHC cells treated with different treatments detected by RT-qPCR (n = 3). G) Immunofluorescence staining of TNF-α in FHC cells after different treatments (scale bar = 10 μm). H-I) Immunofluorescence staining of iNOS and CD206 in RAW264.7 cells after different treatments (scale bar = 10 μm). J) Western blot analysis of the expression of E-cadherin, Occludin, Bcl2, and Bax proteins in FHC cells after different treatments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. one-way ANOVA, Tukey’s multiple comparison test