Fig. 3

Determination of the optimal dosage of PB nanozymes for the treatment of UC model in mice. A) Schematic representation of the experimental protocol employed for UC treatment using PB nanozymes. B) Changes in body weight following various treatments. C) Disease Activity Index (DAI) changes after different treatments. D) Representative images illustrating the macroscopic appearance and H&E staining of colon tissue after different treatments (scale bar = 200 μm). E) Colon length (n = 5). F-H) The expression of mRNAs for TNF-α, IL-1β, and IL-6 was determined by RT-qPCR after different treatments (n = 4). I) The expression of proteins for Occludin, Bcl2, and Bax was analyzed by Western blot after different treatments. ns, not significant. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. one-way ANOVA, Tukey’s multiple comparison test