Fig. 1

Characterization of NEs (CUR-NE and DF-NE). (A) Overlay of average size distribution by intensity measured on Day 0. (B) TEM image of CUR-NE. Magnification = 71kX, Scale bar = 100 nm. (C) Size of NEs stored for 72 h at 37 °C in high (20%) bovine serum-containing culture media. (D) Size of NEs before and after centrifugation at 3000 rpm for 30 min. (E) NIRF signal intensity comparison performed at the same settings between CUR-NE and DF-NE. (F) Long-term size stability monitoring of NEs was performed over three months. Each column in bar graph represents the mean ± SD (n = 3). unpaired t-test, ns not significant