Fig. 3

Macrophage uptake and changes in CD86 expression. (A) Representative images of RAW 264.7 macrophages exposed to 40 \(\:\mu\:\)M of DiR-labeled CUR-NE and volume-matched DF-NE for 6 h and imaged for DAPI nuclei staining, Cy7 staining, and overlay of brightfield, DAPI, and Cy7. Scale bar: 20 \(\:\mu\:\)m. (B) Flowcytometry histogram plot comparison of cellular uptake of 40 \(\:\mu\:\)M CUR-NE and volume-matched DF-NE in LPS-activated RAW 264.7 macrophages. The percentage is shown as the mean ± SD. n = 3/group. (C) Representative density plots showing changes in CD86⁺ macrophages in response to treatments. (D) Quantitative changes in CD86⁺ expression in presence of varying doses of CUR-NE, CUR FD (40 \(\:\mu\:\)M) and DF-NE (volume-matched to highest concentration). The data is shown as the mean ± SD. n = 3/group independent cell culture, and 40,000 cells were counted. One-way ANOVA with Tukey’s multiple comparisons test. ns not significant, **p < 0.005, ****p < 0.00005. Data was analyzed using Attune NXT software