Fig. 10

MNM/siRNA NPs administration attenuated MIRI and improved cardiac function. (a) Schematic illustration of the intervention process. (b) Representative images of Evan’s blue and TTC stained hearts from mice 24 h after MIRI. (c,d) Quantification of the percentage of AAR and IS in hearts from mice 24 h after MIRI (mean ± SD, n = 5). (e) Representative images of Masson’s trichrome stained hearts from mice 14 days after MIRI; scale bar: 500 μm (top) and 50 μm (bottom). (f) Quantification of the percentage of myocardial fibrosis in hearts from mice 14 days after MIRI (mean ± SD, n = 5). (g) Representative images of M-mode echocardiography assessing the cardiac function in mice 14 days after MIRI. (h-k) Quantification of LVEF, LV-FS, LVEDD, and LVESD in hearts from mice 14 days after MIRI (mean ± SD, n = 5). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 (one-way ANOVA with Dunnett’s multiple comparisons test)