Fig. 3

Characterization of formulated MNM/siRNA NPs. (a) Scheme illustrating the preparation of MNM/siRNA NPs. (b,c) Particle size and zeta potential of the MNM, siRNA NPs, and MNM/siRNA NPs (n = 3). (d) Colloidal stability of MNM/siRNA NPs over a span of 96 h in PBS or FBS (n = 3). (e) CLSM images of MNM/siRNA NPs with Dil labeled MNM and Cy5 labeled NPs (Scale bar = 20 μm). (f) TEM images of MNM/siRNA NPs and siRNA NPs (Scale bar = 200 nm). (g) The total protein composition of MNM/siRNA NPs showed by Coomassie blue staining. (h) WB assay of the key proteins on MNM including integrin α9, integrin β1, LFA-1, PSGL-1, HA, CXCR2, and CCR2