Fig. 6

Different concentrations of M1-EVs promote mouse PROM. (A) Representative fluorescence imaging of various M1-EVs in the whole-body and organ tissues from PROM mice. (B) Analysis of the PROM time after different treatments in the mouse model. C and D. The mesenchymal marker (N-cadherin) and epithelial marker (E-cadherin) for native AECs were analyzed by immunofluorescence. Mesenchymal marker (N-cadherin) increased significantly with increasing concentrations of M1-EVs, but the opposite trend was found for the epithelial marker; N = 3; white asterisk: fetal surface of the amnion; scale bar = 200 μm. ns, not significant, *P < 0.05, **P < 0.01