Fig. 7

Verification of D2DR in EGCG@PDA NPs induced protein phosphorylation. A and B are WB results and immunofluorescence localization of boar sperm membrane D2DR receptor; C and D represent the effects of raclopride and EGCG@PDA NPs treatment on P-PKA; E and F represent the effects of raclopride and EGCG@PDA NPs treatment on PTP; G, H, and I represent the effects of raclopride and EGCG@PDA NPs treatment on sperm motility parameters. (n = 10, P < 0.05); J is the proposed schematic of D2DR-mediated regulation mechanism of protein phosphorylation