Fig. 8

The mt-ND3/NADH-CoQ axis is the key target for IL-6-EV to regulate mitochondrial OXPHOS and macrophage polarization. (A) The gene expression of mt-ND3 investigated by qPCR after negative control siRNA (NC-si) and mt-ND3-siRNA (ND3-si) transfection. (B) The protein expression of mt-ND3 investigated by Western blot after ND3-si transfection and (C) statistical analysis. (D-I) The protein expression of ETC Complexes I-V investigated by Western blot after ND3-si transfection and statistical analysis. (J-L) The expression of CD86 and CD206 detected by immunofluorescence after ND3-si transfection and statistical analysis. (M) Effect of ND3-si on NADH-CoQ reductase activity. (N) Effect of ND3-si on ATP production. All data are shown as means ± standard deviations (n = 3), *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001