Fig. 5

NP-NAR regulates signaling pathways related to lipid metabolism. (A) Heatmap of the relative expression levels of DEGs enriched in the PPAR signaling pathway. Relative mRNA expression of PPARα (B), PPARγ (C), CD36 (D), SREBP-1 C (E), ACC1 (F), and FASN (G) in liver tissue. (H-I) Western blot analysis of hepatic PPAR signaling pathways key proteins expression, β-Actin was used as the loading control. Each value was expressed as the mean ± SD (n = 3). (#) p < 0.05, (##) p < 0.01, and (###) p < 0.001 compared to the NC group. (∗) p < 0.05, (∗∗) p < 0.01, and (∗∗∗) p < 0.001 in relation to the MC group. (Δ) p < 0.05, (ΔΔ) p < 0.01, and (ΔΔΔ) p < 0.001