Fig. 3

IRD@M-mediated cellular uptake, oxidative stress, PDT efficacy, and immune escape. (A) CLSM images of Hela cells co-incubated with ICG, IRD, IRD@M for 1 h, 4 h, and 8 h, scale bar = 25 µm and (B) flow cytometry image. (C) CLSM images of Hela cells after co-incubation with ICG, IRD, IRD@M and ROS generation by laser radiation, scale bar = 50 µm and (D) flow cytometry images. Survival of Hela cells co-incubated by different groups (E) without laser(F)with laser irradiation. (G) CLSM images of live-dead staining, scale bar = 100 µm. (H) Apoptosis assay to detect apoptosis in Hela cells with laser or without laser by Annexin V/PI double labeling method after incubation with different groups. (I) Incubation of ICG, IRD, IRD@M to determine immune escape ability. Scale bar = 20 µm. Data are expressed as mean ± SD (n = 3). ** p < 0.01; and *** p < 0.001