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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: Biomimetic gene delivery system coupled with extracellular vesicle–encapsulated AAV for improving diabetic wound through promoting vascularization and remodeling of inflammatory microenvironment

Fig. 5

Assessment of HUVECs proliferation and migration in co-culture with VEGF-EV-AAV/MSC-Exo@FHCCgel under simulated hyperoxic conditions. (a) Wound scratch assay demonstrating the migration of endothelial cells (ECs). (b) Confocal laser scanning microscopy (CLSM) images depicting the transfection of endothelial cells with F-actin/DAPI staining to evaluate cell number and morphology. (c) CLSM imaging for the assessment of intracellular reactive oxygen species (ROS) in ECs pre- and post-incubation with H₂O₂ across various treatments. (d) Relative expression levels of vascular endothelial growth factor (VEGF). (e) The corresponding quantification of closure rates of wound scratch assay. (f) Quantitative analysis of cell counts of F-actin/DAPI staining. (g) Statistical evaluation of ROS intensity within ECs. (h) EDU level of HUVECs under the oxidative culture conditions described above. (i) The tube formation assay to assess the ability of vascularization promotion, and (j) relative analysis. Error bars represent mean ± standard deviation, with n = 3 experiments conducted. Statistical notations: n.s.=not significant, **p < 0.01, ***p < 0.001, indicating levels of statistical significance

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Journal of Nanobiotechnology

ISSN: 1477-3155

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