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Fig. 7 | Journal of Nanobiotechnology

Fig. 7

From: Biomimetic gene delivery system coupled with extracellular vesicle–encapsulated AAV for improving diabetic wound through promoting vascularization and remodeling of inflammatory microenvironment

Fig. 7

Immunomodulatory Effects and Enhanced Wound Healing Induced by VEGF-EV-AAV/MSC-Exo@FHCCgel in Diabetic Wounds. This formulation ameliorated immune dysregulation and fostered effective wound repair. Western blot analyses were conducted to assess protein levels within (a-b) the cGAS/STING and (c-d) efferocytosis signaling pathways in skin tissues extracted from mice with varied treatments. (e-h) Flow cytometric evaluation and associated quantitative analysis of emerging tissue markers for CD86 (indicative of M1 macrophages) and CD206 (indicative of M2 macrophages) at day 3. (i) Immunofluorescence staining for Foxp3 (a Treg marker) on days 14 and 28, accompanied by (j) corresponding statistical analysis. (k-l) Flow cytology sorted CD25+/Foxp3+ cells picked, and relative analysis. (m-p) Quantitative PCR was employed to measure transcript levels of inflammatory mediators in wound tissues on day 7 post-treatment. Error bars represent the mean ± standard deviation, with n = 3. Statistical significance is denoted by **p < 0.01, ***p < 0.001, and n.s.=not significant

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Journal of Nanobiotechnology

ISSN: 1477-3155

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