Fig. 4

In Vitro Biocompatibility of C-GAP and Its Enhancement of Radiotherapy Efficacy. A, B Representative Images and Semi-Quantitative Analysis of Cytotoxicity of 0–20 μg/ml C-GAP on SCC-VII Cells Observed under Confocal Microscopy After Live/Dead Cell Staining (n = 5, one-way ANOVA test, green fluorescence represents SYTO9 for live cells, red fluorescence represents PI for dead cells). C CCK-8 Assay to Assess the Effect of 0–20 μg/ml C-GAP on the Proliferation Activity of SCC-VII Cells (n = 5, one-way ANOVA test). D–E Representative Images and Semi-Quantitative Analysis of the Impact of 0–20 μg/ml C-GAP on the Growth of SCC-VII Cells Using the Cell Cloning Formation Test (n = 5, one-way ANOVA test). F–H Representative Images and Semi-Quantitative Analysis of the Killing Effect of 1 μg/ml C-GAP Combined with 0–16 Gy Radiotherapy on SCC-VII Cells Observed under Confocal Microscopy After Live/Dead Cell Staining (n = 5, one-way ANOVA test, green fluorescence represents SYTO9 for live cells, red fluorescence represents PI for dead cells). I CCK-8 Assay to Evaluate the Effect of 1 μg/ml C-GAP Combined with 0–16 Gy Radiotherapy on the Proliferation Activity of SCC-VII Cells (n = 5, one-way ANOVA test). J-K Representative Images and Semi-Quantitative Analysis of the Impact of 1 μg/ml C-GAP Combined with 0–16 Gy Radiotherapy on the Growth of SCC-VII Cells Using the Cell Cloning Formation Test (n = 5, t-test). The data are presented as the means ± SDs. ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001