Fig. 5

In Vitro Experiments on C-GAP-Induced ICD in OSCC Cells. A–D Representative Images and Semi-Quantitative Analysis of CRT and HMGB1 Expression in SCC-VII Cells from Different Groups Detected by Immunofluorescence Staining (n = 5, one-way ANOVA test). E Representative Images of Western Blotting Analysis of CRT and HMGB1 Expression in SCC-VII Cells from Different Groups (n = 5). F Flow Cytometry Detection of ROS Levels in SCC-VII Cells from Different Groups. G Microplate Reader Measurement of ATP Levels in SCC-VII Cells from Different Groups (n = 5, one-way ANOVA test). The data are presented as the means ± SDs. ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001