Fig. 2

The antitumor efficacy of DOX-SP in vitro. (A) Cell viability of N1S1 cells and McA-RH7777 cells after incubation with series concentrations of SP, DOX and DOX-SP for 24 h. (B) Flow cytometry results of N1S1 cells apoptosis after different treatments for 24 h. (C) Quantification of cell apoptosis rate in (B). Data are presented as means ± SD. (D) Fluorescence microscopic images of McA-RH7777 cells costained with calcein AM (green, live cells) and propidium iodide (red, dead cells) after being incubated with SP, DOX and DOX-SP for 24 h. Scale bars = 100 μm. A and C were analyzed for group differences using a one-way ANOVA, with a p-value < 0.05 indicating significant differences