Fig. 1

Physiochemical characterization of the nanoparticles. (a) Schematic illustration of the composition of N-7DHC-lipos. PC, phosphatidylcholine. (b) Cryo-TEM images of 7DHC-lipos and N-7DHC-lipos. Scale bars, 50 μm (upper) and 200 μm (lower). (c) Size distribution of 7DHC-lipos and N-7DHC-lipos, based on cyro-TEM results and analysis of more than 50 nanoparticles by Image J. (d) Hydrodynamic sizes of 7DHC-lipos and N-7DHC-lipos, measured by DLS in 1×PBS. (e) Zeta potential of 7DHC-lipos and N-7DHC-lipos. (f) Table summering sizes and zeta potentials of 7DHC-lipos and N-7DHC-lipos. (g) Hydrodynamic size change of 7DHC-lipos under different temperatures (4 °C, 25 °C, 37 °C) and pH values (pH 5.5, pH 6.5, pH 7.4) over two weeks incubation in 1×PBS. (h) 7DHC release from 7DHC-lipos, measured in buffer solutions with pH 7.4, 6.5, and 5.5 at 37 °C (n = 3 independent experiments). Data are presented as mean ± SD